Kawasaki Heavy Industries began research on automated cell cultivation equipment in the mid-2000s. So in 2009, Kawasaki Heavy Industries developed the first automated human iPS cell cultivation equipment in the world, AUTO CULTURE. For human iPS cells to be adopted practically upon development of medical treatments as well as drugs there is an urgent need to establish an efficient cultivation method. Furthermore, before human iPS cells differentiate into various cells such as organs or nerves, even the slightest change in temperature or air pressure can cause them to start differentiating earlier than normal.

Not only must specific temperatures be maintained and contamination by bacteria prevented, but it is in addition necessary to conduct delicate operations by hand such as injecting a precise amount of fluid with a pipette when the culture fluid is changed. Developing cell cultures is by nature an extremely delicate process. Yet, there still are not nearly enough human iPS cells for such research use. Since then, many human iPS cells have been cultured and provided to researchers around the world, to be used upon inducing differentiation into specific organs for example. Human iPS cells first attracted huge international attention when in 2007 Professor Shinya Yamanaka of Kyoto University became first in the world to develop a technique for their production from human skin. They are expected to be useful in discovering the cause of diseases and developing new drugs, as well as for cell transplantation and other such regenerative medical treatments. Human iPS cells are pluripotent cells, capable of becoming a wide variety of cells or tissues for human body parts such as nerves, cardiac muscles, livers or pancreases. In the future, hydrogen peroxide will be used for sterilization, enabling cell cultivation for use in regenerative medicine. Virus infections as potential targets of preventive treatments for type 1 diabetes. Expression of toll-like receptors in the pancreas of recent-onset fulminant type 1 diabetes. Shibasaki S, Imagawa A, Tauriainen S, Iino M, Oikarinen M, Abiru H, et al. Fulminant type 1 diabetes in Korea: high prevalence among patients with adult-onset type 1 diabetes.

Epub 9.Ĭho YM, Kim JT, Ko KS, Koo BK, Yang SW, Park MH, et al. Fulminant type 1 diabetes: a nationwide survey in Japan. Imagawa A, Hanafusa T, Uchigata Y, Kanatsuka A, Kawasaki E, Kobayashi T, et al. Nature clinical practice Endocrinology & metabolism. Fulminant type 1 diabetes: a novel clinical entity requiring special attention by all medical practitioners. Polyinosinic:polycytidylic acid transfection can mimic viral infection, and Exendin-4 exerted an anti-apoptotic effect both in MIN6 and insulin-producing cells from human induced pluripotent stem cells. Glucagon-like peptide-1 receptor, protein kinase A, and phosphatidylinositol-3 kinase inhibitors counteracted the anti-apoptotic effect of Exendin-4. Exendin-4 treatment suppressed the elevated gene expression levels and reduced polyinosinic:polycytidylic acid-induced apoptosis both in MIN6 cells and in insulin-producing cells from human induced pluripotent stem cells.

Polyinosinic:polycytidylic acid transfection led to elevated expression of the genes encoding IFNα, IFNβ, CXCL10, Fas, viral receptors, and IFN-inducible antiviral effectors in MIN6 cells. The anti-apoptotic effect of Exendin-4 was also evaluated. Gene expression was analyzed by real-time PCR, and apoptosis was evaluated by caspase-3 activity and TUNEL staining. Polyinosinic:polycytidylic acid was transfected into MIN6 cells and insulin-producing cells differentiated from human induced pluripotent stem cells via small molecule applications. The aim of this study was to investigate the acceleration and protective mechanisms of beta cell destruction by establishing a model of viral infection in pancreatic beta cells.

Viral infection is associated with pancreatic beta cell destruction in fulminant type 1 diabetes mellitus.

Human japanese ips